Journal: STAR Protocols

Article Title: A Protocol for a Novel Human Ex Vivo Model of Aneurysm

doi: 10.1016/j.xpro.2020.100108

Figure Lengend Snippet: Effect of Ang II Administration on Arterial VSMC αSMactin and ER-TR7 Immunopositivity Representative images of immunohistochemistry for (A and B) alpha-smooth muscle actin (αSMactin; green color) alongside a DAPI nuclear label (blue), or (C and D) ER-TR7 (green color) alongside a DAPI nuclear label (blue), in sections from human umbilical cord arteries after insertion within a bioreactor for 72 h without Ang II (untreated; A and C) and with Ang II (Ang II; B and D). Scale bar in (A) represents 50 μm and is applicable to all panels.

Article Snippet: Monoclonal anti-ER-TR7 antibody , Bio-Techne , Cat#NB100-64932.

Techniques: Immunohistochemistry

Journal: STAR Protocols

Article Title: A Protocol for a Novel Human Ex Vivo Model of Aneurysm

doi: 10.1016/j.xpro.2020.100108

Figure Lengend Snippet:

Article Snippet: Monoclonal anti-ER-TR7 antibody , Bio-Techne , Cat#NB100-64932.

Techniques: Recombinant, Modification, Staining, Blocking Assay, Plasmid Preparation, Software

Journal:

Article Title: NKX2.3 is required for MAdCAM-1 expression and homing of lymphocytes in spleen and mucosa-associated lymphoid tissue

doi: 10.1093/emboj/19.9.2015

Figure Lengend Snippet: Fig. 2. Spleen from NKX2.3 mutants lacks the typical marginal zone. Cryostat sections from adult wild-type (A, C, E and G) and mutant (B, D, F and H) spleens were immunostained with specific antibodies as indicated: Thy 1 (blue) for T cells, IgM (red) and IgD [green in (A and B)] for B cells, and MOMA1 [green in (C and D)] for metallophilic macrophages. Note the absence of marginal zone B cells (B) and metallophilic macrophages (D) in mutants compared with wild type (A and C). Also note the mixing of B and T cells in mutant spleen. ER-TR-9 antibody (red) detects a subpopulation of macrophages in the marginal zone of wild type (E), which is scarce and scattered in mutant spleen (F). ER-TR-7 (red) identifies the reticular meshwork in RP (G), which is disorganized and extends into the WP in mutant spleen (H).

Article Snippet: Antibodies and flow cytometry The following antibodies and conjugates were used in this study: anti-Ly5 (B220; clone RA3-6B2; Caltech), anti-CD4 (L3-T4; clone YTS 191.1; Caltech), rat anti-mouse-IgM (clone R6-60.2; PharMingen), anti-Thy1.2 (Becton Dickinson), anti-mouse-IgM–FITC (clone LO-MM-9; Biosource), anti-mouse-IgD–FITC (clone SBA 1; Southern Biotechnology), anti-MAdCAM-1 (clone MECA-89; PharMingen), anti-PNAd carbohydrate epitope (clone MECA-79; PharMingen), anti-MZ-macrophages (clone ER-TR 9; Dianova), anti-metallophile macrophages (clone MOMA-1; Dianova), anti-β7 integrin and anti-ER-TR 7 (BMA Biomedicals AG, Switzerland).

Techniques: Mutagenesis

Journal: Cell reports

Article Title: Migratory dendritic cells, Group 1 innate lymphoid cells, and inflammatory monocytes collaborate to recruit NK cells to the virus infected lymph node

doi: 10.1016/j.celrep.2018.06.004

Figure Lengend Snippet: A) Confocal microscopy (original magnification 10×) of uLN and dLN at the indicated dpi from mice infected with ECTV-mCherry (red) and stained with CXCL9 Ab (green). LN edges are marked with a dashed line. Arrows at 1 dpi show an area of CXCL9 staining and at 2 dpi an area with ECTV-mCherry infected cells. B) Confocal microscopy (60x original magnification) of a dLN at 2 dpi stained with PNAd Ab, an HEV marker (green) and CXCL9 Ab (red). C) As in B but stained with ER-TR7 Ab, an FRC/stroma marker (red), and CXCL9 Ab (green). Scale bars represent 100 μM. Experiment was performed twice with n=3 in each experiment and the results were comparable.

Article Snippet: uLN and dLN were harvested at different dpi, fixed in paraformaldehyde, dehydrated, embedded in OCT-freezing media (Tissue-Tek), cut into 8µ frozen sections, stained with anti-CXCL9 (R&D), anti-PNAD (MECA-79, BD) or anti-ER-TR7 (Santa Cruz).

Techniques: Confocal Microscopy, Infection, Staining, Marker

Journal: Blood

Article Title: Platelets support a protective immune response to LCMV by preventing splenic necrosis

doi: 10.1182/blood-2011-08-376822

Figure Lengend Snippet: Organization of the splenic structure is disrupted by the LCMV infection in platelet-depleted mice. Four groups of mice were treated with PBS or 40 μg of anti-GPIIb antibody 12 hours before, and 2 and 4 days after LCMV Armstrong infection or PBS injection (group 1, nontreated and noninfected; group 2, treated and noninfected; group 3, nontreated and infected; and group 4, treated and infected). Spleens were collected from mice killed 8 days later and thin tissue sections were stained for light or immunofluorescence microscopy analysis. Figure shows representative photomicrographs of H&E (40×, top row); T, B, and DCs (Thy1.2, B220, CD11c; 50×, second row); platelets (β3 integrin; 50×, third row); or fibroblastic reticular cells and LCMV (ER-TR7 and anti-LCMV, 50×, bottom row; n = 5).

Article Snippet: Cryostat sections (6 μm) were fixed in ice-cold acetone, air dried and stained with monoclonal antibodies to F4/80, B220, Thy1.2, and CD11c from eBioscience, to ER-TR9, ER-TR7, and MOMA-1 from Acris GmbH, and to β3 integrin from BD Pharmigen.

Techniques: Infection, Injection, Staining, Immunofluorescence, Microscopy